Journal: Exploration

Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage

doi: 10.1002/exp2.70233

Figure Lengend Snippet: Therapeutic effect of LAC in the monoiodoacetate mouse model. (A) H&E staining of the joint from MIA mice after different treatments. (B) Quantitative analysis of the synovitis score. (C) Representative images of SO&FG‐stained knee joint sections from MIA mice with different treatments. (D) The severity of OA‐like lesions was analyzed using the OARSI scoring system. (E) Immunofluorescence imaging of iNOS (red)/CD206 (green) within the knee joints of mice with different treatments. (F) Corresponding quantification of the MFI of CD206 in the synovium of mice with different treatments. (G) Immunohistochemical staining of COX‐2, TNF‐α, and TRAP in the knee joints of mice with different treatments. (H,I) Quantification of the relative density of COX‐2 + (H) and TNF‐α + (I) cells in the knee joints of mice with different treatments. n = 5 in B and D, and n = 3 in F, H, and I, mean ± SD. The significant differences are determined by one‐way ANOVA analysis with Tukey's multiple comparison test. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns stands for no significant difference.

Article Snippet: Proteins in equivalent amounts were then separated on a 10% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to the PVDF membrane, followed by blocking in TBS‐T solution containing 5% skim milk for 1 h. Subsequently, the membrane was incubated with primary antibody against COX‐2 (66351‐1‐Ig, Proteintech), iNOS (80517‐1‐RR, Proteintech), and CD206 (60143‐1‐Ig, Proteintech) overnight at 4°C.

Techniques: Staining, Analysis, Immunofluorescence, Imaging, Immunohistochemical staining, Comparison

Journal: Frontiers in Microbiology

Article Title: Qijiao Shengbai capsule ameliorates ulcerative colitis in mice by modulating gut microbiota and protecting the intestinal barrier, associated with suppression of the TLR4/MyD88/NF-κB pathway

doi: 10.3389/fmicb.2026.1875650

Figure Lengend Snippet: QJSB alleviates inflammation in DSS-induced UC mice in vivo ( n = 6). (A) , (B) , (C) and (D) Levels of MPO, IL-6, IL-1β, and TNF-α in the colon; (E) , (F) and (G) serum levels of IL-1β, IL-6, and TNF-α; (H) levels of LPS in feces. (I) protein blots for iNOS and COX-2; (J) Relative expression levels of iNOS; (K) relative expression levels of COX-2; (L) correlation analysis between inflammatory mediators and colon-specific metabolites. (### p < 0.001, ## p < 0.01, # p vs. Control; * p < 0.05, ** p < 0.01, *** p < 0.001vs. Model).

Article Snippet: TLR4, MyD88, IκBα, p-IκBα, NF-κB p65, NF-κB p-p65, iNOS, and COX-2 antibodies were obtained from Proteintech (Wuhan, China).

Techniques: In Vivo, Expressing, Analysis, Control